The cardiac work cycle consists of four well-defined isometric and isotonic phases. The IonOptix MultiClamp software enables fast feedback control of pre- and after-load force levels. Together with the IonOptix MyoStretcher OptiForce force transducer and fast piezo motor, we can modulate the force development by rapidly changing the length of the myocyte in response to changing force load. By clamping the diastolic and systolic force levels (pre- and after-load), we can mimic the cardiac cycle, enabling work measurements in single, isolated cardiomyocytes.
Features
Resources
- Best Practices – Measuring Force, Resting Sarcomere Length, and Calcium in Cardiac Slices
- Understanding Advanced Cardiac Tissue Slice Applications
- Work Loop Configuration
- Work Loop Parameters and Units
- Cardiac Tissue Slices: Preparation, Data Acquisition, and Analysis
- Short video: Cardiac Slice Work Loop_4 Hz
- Short video: Cardiac Slice Work Loop_2 Hz
- Short video: Cardiac Slice Work Loop_0.5 Hz
- Preparation of a cardiac slice
- IonOptix Video Tutorials: Murine Cardiomyocyte Isolation
- IonOptix Video Tutorials: CytoSolver Batch Analysis
- IonOptix Video Tutorials: Analyzing Unmarked Events
- IonOptix Video Tutorials: IonWizard Data Analysis
- Workloops Handout
- Techniques and Best Practices for Cardiomyocyte Isolation
Note: This product is intended for research purposes only. It is not certified for clinical applications (including diagnostic purposes). Use of this product in uncertified applications is in violation of FDA regulations.