IonOptix’ FloAcq acquisition and recording module provides real-time indicators of arterial flow dynamics. The endothelial response to flow-induced stress (e.g., the generation and secretion of nitric oxide) is an important modulator of vascular tone and function, linking vasodilation and constriction to the dynamics of fluid flow. IonWizard’s FloAcq acquisition module is the first to perform real-time calculations of important indicators of arterial flow, including shear stress. By acquiring the [...]

MultiAcq gives you the speed and ease of microscope control needed to achieve high throughput in your experiments. With this new interface, you can: Focus on your sample, either manually or automatically Quickly move around the dish using buttons or keyboard shortcuts for maximum speed Digitally rotate, saving time and effort Mark cell position and rotation for repeated measurements [...]

IonWizard integrates the presentation of fluorescence traces and images, analog voltages, and cell length data under an intuitive interface. It analyzes raw data by performing ratio, ion, or linear calibration calculations. Fluorescence calculations include a comprehensive selection of background subtraction techniques.  Specialized functions such as trace averaging, composite images, and transient analysis eliminate common extra work. Clipboarding and exporting of trace and image data eases integration with other programs. [...]

The cardiac work cycle consists of four well-defined isometric and isotonic phases. The IonOptix MultiClamp software enables fast feedback control of pre- and after-load force levels. Together with the IonOptix MyoStretcher OptiForce force transducer and fast piezo motor, we can modulate the force development by rapidly changing the length of the myocyte in response to changing force load. By clamping the diastolic and systolic force levels (pre- and after-load), [...]

The PMT Acquisition Add-on module (PMTAcq) extends the IonWizard module into a full-featured ratiometric PMT acquisition system while allowing access to the analysis features of IonWizard during data collection. PMTAcq integrates seamlessly with the IonOptix PMT Sub-System to provide a coherent photometry solution. It provides integrated support for continuous ratiometric sampling using any IonOptix dual-excitation light source (HyperSwitch or µStep). PMTAcq displays raw and background-subtracted counts. For single wavelength [...]

SarcLen enables IonWizard to acquire high resolution sarcomere length measurements. The accompanying image to the right shows IonWizard during data acquisition as extended by SarcLen. SarcLen performs an average of the lines within the area of interest to create a well resolved striation pattern (grey). A fast Fourier transform (FFT) is then calculated and the peak within the power spectrum (red) represents the average sarcomere spacing, measured in real [...]