IonWizard integrates the presentation of fluorescence traces and images, analog voltages, and cell length data under an intuitive interface. It analyzes raw data by performing ratio, ion, or linear calibration calculations. Fluorescence calculations include a comprehensive selection of background subtraction techniques. Specialized functions such as trace averaging, composite images, and transient analysis eliminate common extra work. Clipboarding and exporting of trace and image data eases integration with other programs. Extensive use of individual user templates and context-sensitive online help increases productivity.
Features
- Data Acquisition. Data acquisition via the SarAcq, EdgAcq, VesAcq, FloAcq and PMTAcq DLLs.
- Signal Generator w/ optional Advanced Signal Generator. Send control voltages through the interface to drive external devices.
- Trace Display/Printing. Display multiple traces. Zoom-in on small details and scroll over whole trace. Print traces at any zoom level.
- Trace Analysis. Background subtraction, ratio and ion calculation. Analog and cell length calibrations. Digital trace filtering.
- Annotation. Mark experimental events, and make experimental notes both during acquisition and off-line.
- Trace Averaging. Average trace data via several methods to remove random noise from repetitive signals.
- Single File. All data, including images, is stored in a single data file for easy handling and rapid access.
- Transient Analysis. Automatic or manual marking of important data transients. Analyze transients to extract important parameters (baseline, peak value, etc.).
- Image Support. Subtract backgrounds, calculate ratio and ion images, and mask out non-fluorescent background data. Move forward or backward through image sequences with the movie player. Images are time synchronized to pixel average traces. Make composite arrays of select images.
- Clipboard/Export Support. Copy traces, single images or composite images to clipboard for figure making in third party graphics packages. Export trace data in ASCII format for analysis in third party data analysis applications.
Resources
- Best Practices – Measuring Force, Resting Sarcomere Length, and Calcium in Cardiac Slices
- Porcine Isolation Protocol
- Understanding Advanced Cardiac Tissue Slice Applications
- Calcium Indicator Dye Loading in Cardiac Slices
- Work Loop Configuration
- IonOptix Video: Primary and Cultured Cardiomyocyte Contractility Algorithms
- IonOptix Video Tutorials: CytoSolver 3 Fitting Paced and Unpaced Cardiomyocytes
- Calcium and contractility best practices
- CytoSolver 3.0 Batch Analysis Guide
- CytoSolver 3 analysis of paced cardiomyocytes
- CytoSolver 3 analysis of unpaced iPSC-CM
- Cardiac Tissue Slices: Preparation, Data Acquisition, and Analysis
- IonOptix Video Tutorials: Operation of the C-Pace EM Culture Stimulator
- Short video: Cardiac Slice Work Loop_4 Hz
- Short video: Cardiac Slice Work Loop_2 Hz
- Short video: Cardiac Slice Work Loop_0.5 Hz
- Preparation of a cardiac slice
- Functional Characterization of Excitation-Contraction Coupling in Cardiomyocytes
- Excitation-Contraction Coupling: Simultaneous Measures of Intracellular Calcium & Action Potentials
- Measuring iPSC-CM Calcium & Contractility
- Simultaneous Action Potential, Calcium, and Contractility Case Study
- Removal of papillary muscles from a rat heart
- Intact Muscle Chamber Manual
- IonOptix Video Tutorials: Murine Cardiomyocyte Isolation
- IonOptix Video Tutorials: IonWizard Experiment Design
- IonOptix Video Tutorials: Calcium & Contractility System Acquisition Quick Start
- IonOptix Video Tutorials: CytoSolver Batch Analysis
- IonOptix Video Tutorials: Analyzing Unmarked Events
- IonOptix Video Tutorials: IonWizard Data Analysis
- Transient Analysis Features
- Mutech and RTD card installation for IW6
- IonWizard Core and Analysis Manual
- IonWizard Acquisition Manual
- G protein-coupled receptor kinase 2 ablation in cardiac myocytes before or after myocardial infarction prevents heart failure.
- Techniques and Best Practices for Cardiomyocyte Isolation
- Maturation of intracellular calcium homeostasis in sheep pulmonary arterial smooth muscle cells.
- Late exercise training improves non-uniformity of transmural myocardial function in rats with ischaemic heart failure.
Note: This product is intended for research purposes only. It is not certified for clinical applications (including diagnostic purposes). Use of this product in uncertified applications is in violation of FDA regulations.