The PMT Acquisition Add-on module (PMTAcq) extends the IonWizard module into a full-featured ratiometric PMT acquisition system while allowing access to the analysis features of IonWizard during data collection. PMTAcq integrates seamlessly with the IonOptix PMT Sub-System to provide a coherent photometry solution. It provides integrated support for continuous ratiometric sampling using any IonOptix dual-excitation light source (HyperSwitch or µStep). PMTAcq displays raw and background-subtracted counts. For single wavelength dyes such as Fluo-3/4, PMTAcq also normalizes values to provide F0. For ratiometric dyes such as Fura-2 and Indo-1, PMTAcq performs real-time ratios as well as calibrated Ca2+.
Resources
- Best Practices – Measuring Force, Resting Sarcomere Length, and Calcium in Cardiac Slices
- Porcine Isolation Protocol
- Understanding Advanced Cardiac Tissue Slice Applications
- Calcium Indicator Dye Loading in Cardiac Slices
- Cardiac Tissue Slices: Preparation, Data Acquisition, and Analysis
- Preparation of a cardiac slice
- Excitation-Contraction Coupling: Simultaneous Measures of Intracellular Calcium & Action Potentials
- IonOptix Video Tutorials: Murine Cardiomyocyte Isolation
- IonOptix Video Tutorials: IonWizard Experiment Design
- IonOptix Video Tutorials: Calcium & Contractility System Acquisition Quick Start
- IonOptix Video Tutorials: CytoSolver Batch Analysis
- IonOptix Video Tutorials: Analyzing Unmarked Events
- IonOptix Video Tutorials: IonWizard Data Analysis
- Transient Analysis Features
- Techniques and Best Practices for Cardiomyocyte Isolation
Note: This product is intended for research purposes only. It is not certified for clinical applications (including diagnostic purposes). Use of this product in uncertified applications is in violation of FDA regulations.