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InsideAnswers: Techniques and Best Practices for Cardiomyocyte Isolation Q&A
In this Q&A video series sponsored by IonOptix, experts address your best questions and greatest challenges with regards to cardiomyocyte isolation, purification, culture and analysis. These questions have been adapted from registrants’ input during our November 2018 webinar, Techniques and Best Practices for Cardiomyocyte Isolation.
Presenters
Professor of Medicine
Cardiac Muscle Research Laboratory
Stanford University School of Medicine
rliao7@stanford.edu
Lecturer of Cardiovascular Sciences
College of Medical and Dental Sciences
University of Birmingham
D.Pavlovic@bham.ac.uk
Post Doctoral Fellow
Yong Loo Lin School of Medicine
National University of Singapore
mdcmaa@nus.edu.sg
Discussion Topics:
- Strategies to consistently improve your isolation success
- Tips for getting started with the Langendorff-free method of cardiomyocyte isolation
- Ideal short- and long-term culture conditions
- Which enzymes to use and how to ensure the heart is optimally digested
Associated Resources:
- Ackers-Johnson, M. et al (2016). A Simplified, Langendorff-Free Method for Concomitant Isolation of Viable Cardiac Myocytes and Nonmyocytes From the Adult Mouse Heart
- Liao Lab Protocol: Rat Cardiomyocyte Isolation Using non-Langendorff Mode
- Liao Lab Protocol: Rat Cardiomyocyte Isolation Using Langendorff Mode
- Liao Lab Protocol: Adult Mouse Cardiomyocyte Culture Procedure
