Live Demonstration
Best Practices: Measuring Force, Resting Sarcomere Length, and Calcium in Cardiac Slice Preparations
In this online demonstration, Dr. Adam Veteto shows how to successfully acquire force, resting sarcomere length, and calcium data from cardiac slices, including tips and best practices.
Unlike isolated cardiomyocytes, cardiac tissue slices better preserve native myocardial tissue, including both fibroblasts and myocytes. And unlike whole heart studies, slices are better suited for acquisition of functional parameters like calcium transients. Unlike both models, however, cardiac slices have not been widely utilized.
Key topics:
- How to maximize force development in cardiac slices.
- How and why to acquire resting sarcomere length.
- How to best load calcium indicators in cardiac slice and optimizing fluorescence signal-to-noise ratio (SNR).
*This demo will be exclusively available to registrants who attend the live event. Register today to save your spot!
To register, click the registration button above or go to: https://bit.ly/3WuFdq
About the speaker:
Adam Veteto, PhD
Applications Scientist/Visiting Scientist
Medical Pharmacology and Physiology
IonOptix/University of Missouri
Dr. Adam Veteto joined IonOptix as an Applications Scientist in 2019 after completing his PhD in Medical Pharmacology and Physiology at the University of Missouri. He continues collaborating with investigators at MU to elucidate mechanisms underlying changes in cardiac calcium homeostasis with aging due to mechanical perturbation.